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upright microscope (electrophysiology)  (Scientifica)

 
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    Structured Review

    Scientifica upright microscope (electrophysiology)
    Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the <t>electrophysiology</t> setup <t>microscope.</t> Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.
    Upright Microscope (Electrophysiology), supplied by Scientifica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/upright microscope (electrophysiology)/product/Scientifica
    Average 90 stars, based on 1 article reviews
    upright microscope (electrophysiology) - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures"

    Article Title: Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.103915

    Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the electrophysiology setup microscope. Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.
    Figure Legend Snippet: Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the electrophysiology setup microscope. Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.

    Techniques Used: Microscopy



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    Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the <t>electrophysiology</t> setup <t>microscope.</t> Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.
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    Image Search Results


    Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the electrophysiology setup microscope. Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.

    Journal: STAR Protocols

    Article Title: Protocol for generating and using human iPSC-derived microglia-containing air-liquid-interface cortical organoid cultures

    doi: 10.1016/j.xpro.2025.103915

    Figure Lengend Snippet: Electrophysiological recording of MG-ALI-CO (A) Schematic overview of the different steps preceding and following electrophysiological recording. Created with BioRender.com. (B) MG-ALI-CO bright-field image as seen from the electrophysiology setup microscope. Scale bar, 50 μm. (C) Representative trace of an individual neuron’s electrophysiological recording. (D) Quantification of sEPSC amplitude and frequency from individual neurons in DIV120-170 MG-ALI-CO (6 weeks after MacPre integration; 10 5 integrated MacPre). Data are represented as means + SEM, N=12 cells (1 cell = 1 MG-ALI-CO). (E) MG-ALI-CO immunostained for patched neurons (biocytin) and IBA1. DAPI stains nuclei. Boxed areas are shown at higher magnification in the two right panels and show the close proximity of biocytin-filled neurons and microglia. Scale bars, 50 μm/ 10 μm.

    Article Snippet: Upright Microscope (electrophysiology) , , SliceScope Pro 6000 (Scientifica).

    Techniques: Microscopy